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Exploration of annealing temperatures.

By Hirokazu Toju (316083), Akifumi S. Tanabe (316084), Satoshi Yamamoto (316085) and Hirotoshi Sato (117839)


<p>Three ascomycete and four basidiomycete species were subjected to amplification of the (a) ITS1 region (ITS1-F_KYO–ITS2_KYO2), (b) ITS2 region (ITS3_KYO2–ITS4), and the (c) entire ITS region (ITS1-F_KYO2–ITS4) at four annealing temperatures (electrophoresed on 2.2% agarose gels). See <a href="" target="_blank">Table 2</a> for the abbreviations of fungal specimens. N, negative control.</p

Topics: Microbiology, Ecology, annealing
Year: 2013
DOI identifier: 10.1371/journal.pone.0040863.g004
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Provided by: FigShare
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