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NO-dependent effects of leptin on glyceroneogenesis and NOS III phosphorylation in WAT explants from rats.

By Anne-Marie Jaubert (316226), Graziella Penot (316229), Fatoumata Niang (316230), Sylvie Durant (316233) and Claude Forest (316237)

Abstract

<p>Explants from SD (A) or Zucker rats (B, C) were pre-treated or not with L-NAME (1 mmol/L) or AG490 (10 µmol/L) for 30 min, then exposed or not to either leptin (10 µg/L), SNAP (1 mmol/L) or IFN-γ (50 µg/L) for 2 h in KRB medium containing 2% BSA and [1-<sup>14</sup>C]-pyruvate. Glyceroneogenic flux was measured by the [1-<sup>14</sup>C]-pyruvate incorporation into neutral lipids. Each value represents the mean ± SEM, (n = 4) *, <i>P</i><0.01 <i>vs</i>. control; <sup>a </sup><i>P</i><0.01 <i>vs.</i> leptin-treated explants. (C) Representative autoradiogram of a western blot performed on WAT cytosolic proteins from Zucker rats reveals total NOS III and its Ser<sup>1179</sup> phosphorylated form.</p

Topics: Biochemistry, Physiology, leptin, glyceroneogenesis, nos, iii, phosphorylation, wat, explants
Year: 2013
DOI identifier: 10.1371/journal.pone.0040650.g002
OAI identifier: oai:figshare.com:article/281122
Provided by: FigShare
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