<p>TGF-β up-regulated the expression of α-SMA in fibroblasts to promote the differentiation from fibroblast to myofibroblast. In contrast, Ac-SDKP and LY364947 pretreatment prevented the α-SMA and SRF expression. A) α-SMA protein expression and distribution as analyzed by immunohistochemistry; B) Western blot analysis with antibodies against α-SMA and SRF; C) α-SMA protein expression measured by Western blot; D) SRF protein expression measured by Western blot; E) α-SMA mRNA expression measured by Real-time PCR; F) SRF mRNA expression measured by Real-time PCR.</p
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