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Measurement of apoptosis and autophagy in PC-3 cells that received various treatments.

By Hui-Wen Chiu (317073), Wen-Hung Fang (317074), Yen-Lin Chen (317075), Ming-Der Wu (317076), Gwo-Fang Yuan (20820), Sheng-Yow Ho (317077) and Ying-Jan Wang (317078)

Abstract

<p>(A) Early apoptosis, detected using an Annexin V apoptosis detection kit, was measured using flow cytometry. Cells were treated with IR (4 Gy) and MP (25 μM) for 48 hrs. (B) Development of AVOs in PC-3 cells. Detection of green and red fluorescence in AO-stained cells using flow cytometry. (C) Quantification of AVOs with AO-stained cells treated with IR (4 Gy) or MP (25 μM) alone or in combination using flow cytometry. #, <i>p</i><0.05, IR versus combined treatment. *, <i>p</i><0.05, MP versus combined treatment. Data are presented as the mean ± standard deviation of three independent experiments. (D) EM microphotographs of PC-3 cells treated with IR (4 Gy) and MP (25 μM) for 48 hrs. The <i>white arrows</i> point to autophagic vacuoles and autolysosomes. (E) Western blotting for LC3-I, LC3-II, p62/SQSTM1 and Atg5–12 in PC-3 cells. Cells were treated with IR (4 Gy) and MP (25 μM) for 48 hrs.</p

Topics: Cell Biology, Pharmacology, Chemistry, Cancer, apoptosis, autophagy, pc-3, cells, received
Year: 2013
DOI identifier: 10.1371/journal.pone.0040462.g003
OAI identifier: oai:figshare.com:article/284774
Provided by: FigShare
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