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Uptake of α-Gal A by human kidney ECs.

By Thaneas Prabakaran (317599), Rikke Nielsen (317601), Simon C. Satchell (140614), Peter W. Mathieson (162070), Ulla Feldt-Rasmussen (317604), Søren S. Sørensen (317606) and Erik I. Christensen (317608)

Abstract

<p>(A) Dual immunofluorescence staining showing α-Gal A in the glomerulus (G) in a renal biopsy from a Fabry patient who was infused with α-Gal A 2 h before the biopsy. Localization of α-Gal A is observed in the GECs as indicated by co-localization with CD34 (endothelial cell surface marker). Merged high power view demonstrates that α-Gal A is seen in GECs (white arrowheads) and in podocyte (blue arrowhead) in a human glomerulus from a Fabry disease patient. Labeling for α-Gal A is also seen in the proximal tubule (PT). Scale bar, 25 µm. (B) Peroxidase staining showing localization of α-Gal A (green arrowheads) in larger vessel ECs in a renal biopsy from a Fabry patient who was infused with α-Gal A 2 h before the biopsy. Scale bar, 25 µm. (C) Uptake of Alexa-Fluor 546-labeled α-Gal A (red) in cultured human GECs as a function of time at 37°C. At the indicated times, the cells were fixed and analyzed by confocal microscopy. Scale bar, 5 µm. (D) Co-localization (yellow) of α-Gal A (red) and lysosomes (green). A merged image is shown. Scale bar, 5 µm.</p

Topics: Biochemistry, Cell Biology, Biotechnology, Marine Biology, kidney
Year: 2013
DOI identifier: 10.1371/journal.pone.0039975.g001
OAI identifier: oai:figshare.com:article/286160
Provided by: FigShare
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