<p>(<b>A–B</b>) Compared with 1 year-old littermate controls (<b>A</b>), hematoxylin and eosin (H&E)-stained ileal epithelia, along with underlying lamina propria and muscular layers, appeared normal in 1 year-old <i>Villin-Klf5</i> mice (<b>B</b>). Findings were similar in duodeum, jejunum, and colon of <i>Villin-Klf5</i> mice. Scale bars: 50 µm. (<b>C–D</b>) Quantitation of BrdU-labeled (<b>C</b>) or Ki-67 positive (<b>D</b>) cells revealed no changes in proliferation in the small intestine or colon of <i>Villin-Klf5</i> mice at 1 year of age, compared to littermate controls. (<b>E</b>) The number of chromogranin-A positive enteroendocrine cells per crypt-villus unit was unchanged between control and <i>Villin-Klf5</i> mice at 1 year of age. (<b>F</b>) By Alcian blue staining, no difference was observed in the number of goblet cells within small intestine or colon of 1 year-old control and <i>Villin-Klf5</i> mice. Similar results were observed for proliferation and differentiation of intestinal cell types in 1 month-, 3 month-, and 6 month-old mice. D = Duodenum, J = Jejunum, I = Ileum, C = Colon.</p
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