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Effect of APOBEC3s on ΔVif HIV-1 DNA synthesis.

By Silvia Sanchez-Martinez (217122), Amanda L. Aloia (217099), Demetria Harvin (321148), Jane Mirro (321149), Robert J. Gorelick (14384), Patric Jern (81078), John M. Coffin (182188) and Alan Rein (14387)

Abstract

<p>293-mCAT1 cells were infected with ΔVif HIV-1. Infectivity and RT activity were assayed as described <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0038190#pone.0038190-Derse1" target="_blank">[25]</a>, <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0038190#pone.0038190-Gorelick1" target="_blank">[29]</a>, <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0038190#pone.0038190-Gorelick2" target="_blank">[30]</a>. Twenty-four hours after infection, the cells were lysed and assayed by real-time PCR for <i>Luciferase</i> DNA (black and green lines) as described in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0038190#s4" target="_blank">Materials and Methods </a><a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0038190#pone.0038190-Gherghe1" target="_blank">[32]</a>. Specific infectivity is represented with red and blue lines.</p

Topics: Biophysics, Biochemistry, Physics, Genetics, Molecular Biology, Cancer, Hematology, Virology, apobec3s, hiv-1, dna
Year: 2013
DOI identifier: 10.1371/journal.pone.0038190.g005
OAI identifier: oai:figshare.com:article/302103
Provided by: FigShare
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