Effect of APOBEC3s on ΔVif HIV-1 DNA synthesis.

Abstract

<p>293-mCAT1 cells were infected with ΔVif HIV-1. Infectivity and RT activity were assayed as described <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0038190#pone.0038190-Derse1" target="_blank">[25]</a>, <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0038190#pone.0038190-Gorelick1" target="_blank">[29]</a>, <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0038190#pone.0038190-Gorelick2" target="_blank">[30]</a>. Twenty-four hours after infection, the cells were lysed and assayed by real-time PCR for <i>Luciferase</i> DNA (black and green lines) as described in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0038190#s4" target="_blank">Materials and Methods </a><a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0038190#pone.0038190-Gherghe1" target="_blank">[32]</a>. Specific infectivity is represented with red and blue lines.</p