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Distribution of neoblasts in <i>E. japonensis</i> and their absence in <i>E. buchholzi</i>.

By Maroko Myohara (322904)


<p>(A–G) Intact <i>E. japonensis</i> worms were stained with methyl green-pyronin (A–C), thionine (D–E), or propidium Iodide (F–G). Neoblast pairs (arrows) are localized on the intersegmental septa along the ventral nerve cord in all segments except for the eight anterior-most segments, i.e. the seven head-specific segments (segment I–VII) and the first trunk segment (segment VIII). (H–J) Neoblasts are absent from the corresponding position (arrowheads) in <i>E. buchholzi</i> as revealed by staining with methyl green-pyronin (H), thionine (I), or propidium Iodide (J). (K–N) Behavior of neoblasts (arrows) during regeneration was examined in <i>E. japonensis</i> using methyl green-pyronin staining. Soon after fragmentation, neoblasts in the segment(s) at the ends of a fragment divide (K, large arrowheads) and both daughter cells migrate together (L, large arrowheads) leaving their site of previous occupancy vacant (M, small arrowheads). The recovery of neoblasts occurs after regeneration has completed, except for in the anterior-most segment, i.e., the new segment VIII in which neoblasts are absent (N, small arrowheads). g, gut; gz, growth zone; pr, prostomium; py, pygidium; sg, septal (pharyngeal) gland; vnc, ventral nerve cord. Scale bar, 100 µm for (A–B, D, F, H–J), 5 µm for (C, E, G), and 50 µm for (K–N).</p

Topics: Developmental Biology, neoblasts
Year: 2013
DOI identifier: 10.1371/journal.pone.0037319.g001
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