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Interaction of Girdin with Par-3.

By Kei Ohara (323917), Atsushi Enomoto (323918), Takuya Kato (323919), Takahiko Hashimoto (323920), Mayu Isotani-Sakakibara (323921), Naoya Asai (268574), Maki Ishida-Takagishi (323922), Liang Weng (323923), Masanori Nakayama (163148), Takashi Watanabe (22042), Katsuhiro Kato (254792), Kozo Kaibuchi (75662), Yoshiki Murakumo (30871), Yoshiki Hirooka (323924), Hidemi Goto (22971) and Masahide Takahashi (190232)


<p>A. Interaction of exogenously expressed Girdin and Par-3 in HEK293T cells. Total cell lysates (TCL) from HEK293T cells that expressed Girdin-V5 and either myc-GST or myc-Par-3 were immunoprecipitated (IP) with anti-myc antibody, followed by Western blot analyses using the indicated antibodies. myc-Par-3 and Girdin-V5 are indicated by asterisks and stars, respectively. Open arrowhead indicates IgG heavy chain. B, C. Interaction between endogenous Girdin and Par-3. Lysates from HEK293T cells were immunoprecipitated with two anti-Girdin antibodies (B, available from IBL; C, available from R&D Systems) and normal rabbit IgG antibodies, followed by Western blot analysis using the indicated antibodies. Two spliced isoforms of Par-3 are indicated by arrowheads.</p

Topics: Biophysics, Molecular Biology, Physiology, Developmental Biology, girdin
Year: 2013
DOI identifier: 10.1371/journal.pone.0036681.g002
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Provided by: FigShare
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