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Immunoblotting of LC-3 protein in INS-1E cells after 2, 6 and 12 h treatment with various concentrations of palmitate (PA).

By Luisa Martino (324362), Matilde Masini (324363), Michela Novelli (77048), Pascale Beffy (324364), Marco Bugliani (324365), Lorella Marselli (179072), Pellegrino Masiello (77056), Piero Marchetti (179074) and Vincenzo De Tata (324366)

Abstract

<p>α-tubulin was used as a control for loading. Methodological details are described in Materials and <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0036188#s2" target="_blank">Methods</a>. The figure is representative of gels obtained from three separate experiments. From these gels, band intensities were evaluated by densitometric analysis and expressed as the mean ± SEM of the ratio between LC3-II and LC3-I bands (lower graphs). * p<0.01 vs. untreated control; § p<0.01 vs. PA 0.5 mM.</p

Topics: Biochemistry, Physiology, Chemistry, lc-3, ins-1e, cells, 12, concentrations, palmitate
Year: 2013
DOI identifier: 10.1371/journal.pone.0036188.g003
OAI identifier: oai:figshare.com:article/315063
Provided by: FigShare
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