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Aspirin-induced ROS production.

By Haider Raza (324458) and Annie John (324459)

Abstract

<p>Intracellular production of ROS was measured using DCFDA and fluorescence was measured by flow cytometry using Cell Quest software as described in the <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0036325#s2" target="_blank">Materials and Methods</a>. ROS was measured in GSH depleted HepG2 cells treated with different concentrations of aspirin and also after NAC treatment (<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0036325#pone-0036325-g003" target="_blank">Figure 3i</a>). Result expressed is a typical representation of three determinations. % change in ROS production from a typical histogram is presented in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0036325#pone-0036325-g003" target="_blank">Figure 3ii</a>. Asterisks indicate significant difference (P≤0.05) from control values, # indicate significant difference (P≤0.05) from 5 µmol/ml ASA treated group and δ indicate significant difference (P≤0.05) as compared to 10 µmol/ml ASA treated group.</p

Topics: Biochemistry, Cell Biology, Molecular Biology, Physiology, Pharmacology, Biotechnology, Cancer, ros
Year: 2013
DOI identifier: 10.1371/journal.pone.0036325.g003
OAI identifier: oai:figshare.com:article/315409
Provided by: FigShare
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