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Mitochondrial Activity. A

By Brijesh Kumar Singh (327521), Madhulika Tripathi (327522), Bhushan P. Chaudhari (303555), Pramod K. Pandey (327523) and Poonam Kakkar (144347)


<p>and <b>B.</b> %MTT activity as mitochondrial electron flow and UV auto-fluorescence of mitochondria as reduced pyridine nucleotides [NAD(P)H] respectively. <b>C.</b> Mitochondrial membrane potential was assessed using JC-1dye on flow cytometer. In dot plot, red fluorescence of J-aggregates represents polarized mitochondria (in right panel, red colored) whereas green fluorescence of JC-1 monomers represents depolarized mitochondria (left panel, green colored). CCCP (mitochondrial uncoupler) was used as negative control for mitochondrial membrane potential. Level of significance is denoted as **/## <i>P</i><0.01 and ***/### <i>P</i><0.001. *, compared to vehicle control and #, compared to nimesulide stress.</p

Topics: Biochemistry, Cell Biology, Molecular Biology, Chemistry, chemistry, molecular biology, cell biology, Biochemistry
Year: 2013
DOI identifier: 10.1371/journal.pone.0034200.g006
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Provided by: FigShare
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