Mitochondrial Activity. A

Abstract

<p>and <b>B.</b> %MTT activity as mitochondrial electron flow and UV auto-fluorescence of mitochondria as reduced pyridine nucleotides [NAD(P)H] respectively. <b>C.</b> Mitochondrial membrane potential was assessed using JC-1dye on flow cytometer. In dot plot, red fluorescence of J-aggregates represents polarized mitochondria (in right panel, red colored) whereas green fluorescence of JC-1 monomers represents depolarized mitochondria (left panel, green colored). CCCP (mitochondrial uncoupler) was used as negative control for mitochondrial membrane potential. Level of significance is denoted as **/## <i>P</i><0.01 and ***/### <i>P</i><0.001. *, compared to vehicle control and #, compared to nimesulide stress.</p