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Effect of isoflurne on mRNA expression of HIF target genes.

By Tomoharu Tanaka (47429), Shinichi Kai (336707), Tomohiro Koyama (336708), Hiroki Daijo (336709), Takehiko Adachi (47430), Kazuhiko Fukuda (47431) and Kiichi Hirota (47433)

Abstract

<p>(A) HIF-1 and HIF-2 have unique, as well as common, target genes. HIF-1 specifically regulates glycolytic genes, including lactate dehydrogenase A (LDHA), phosphoglycerate kinase (PGK), as well as carbonic hydrase-9 (CA IX) whereas HIF-2 exclusively regulates POU transcription factor Oct-4, cyclin D1, and transforming growth factor α (TGF-α). Other hypoxia-inducible genes, such as vascular endothelial growth factor (VEGF), glucose transporter 1 (GLUT1), and EPO are regulated by both HIF-1 and HIF-2. (B, C) 6-week-old BALB/c mice were exposed to 10% O<sub>2</sub> (hypoxia) for 3 hours with or without 0.5% isoflurane and compared with controls. Control mice were exposed to air without isoflurane (normoxia). (D) 6-week-old BALB/c mice were exposed to 0.5% or 1.0% isoflurane in air for 3 hours. Data are presented as mean ± SD (n = 6). The expression levels of EPO, VEGF, LDHA and GLUT1 were assayed using real-time RT-PCR and normalized to that of 18S and expressed relative to the mean of mice exposed to air without isoflurane (normoxia).</p

Topics: Cell Biology, Genetics, Physiology, isoflurne, mrna, hif
Year: 2013
DOI identifier: 10.1371/journal.pone.0029378.g005
OAI identifier: oai:figshare.com:article/369794
Provided by: FigShare
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