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Effects of U18666A on the intracellular distribution of FC and LDL in HeLa cells and HMDM.

By Emmanuelle Girard (337171), Jean Louis Paul (337172), Natalie Fournier (337173), Philippe Beaune (10848), Ludger Johannes (261979), Christophe Lamaze (259537) and Benoît Védie (337174)

Abstract

<p>HeLa cells and HMDM were respectively incubated for 6 h with 200 µg/mL LDL (A) or 50 µg/ml AcLDL (C) with 3 µg/ml U18666A or without (control) and stained with filipin to detect FC. (B–D) HeLa cells and HMDM were respectively incubated for 6 h with 200 µg/ml DiI-LDL (B) or 50 µg/ml DiI-AcLDL (D) with 3 µg/ml U18666A or without (control) and processed to visualize LDL distribution. Images were obtained using wide-field epifluorescence microscopy.</p

Topics: Biochemistry, Cell Biology, Genetics, Molecular Biology, u18666a, intracellular, fc, ldl, hela, cells
Year: 2013
DOI identifier: 10.1371/journal.pone.0029042.g010
OAI identifier: oai:figshare.com:article/372288
Provided by: FigShare
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