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Expression of PLDs in response to various stresses.

By Jian Zhao (219525), Cunxi Wang (337946), Mohamed Bedair (337947), Ruth Welti (120203), Lloyd W. Sumner (130587), Ivan Baxter (88322) and Xuemin Wang (337948)

Abstract

<p>(A) RNA blotting of <i>PLD</i>α<i>1</i> and <i>PLD</i>γ transcripts in Arabidopsis leaves exposed to different stress treatments. Arabidopsis Col-0 (3–week old) leaves were detached and floated on water without or with indicated concentrations of chemicals, 1% NaCl, 0.6 M mannitol, 0.2 mM AlCl<sub>3</sub>, 0.2 mM CdCl<sub>2</sub>, 2 mM H<sub>2</sub>O<sub>2</sub>, 0.1 mM salicylic acid (SA), 0.1 mM methyl salicylate (MeSA), 0.1 mM abscisic acid (ABA), or 0.1 mM methyl jasmonate (MeJA) in a growth chamber at 22°C. Leaves were collected at the indicated times for RNA extraction and northern blotting with [α-<sup>32</sup>P]-labeled <i>PLD</i>α<i>1</i> or <i>PLD</i>γ<i>1</i> full-length cDNA as a probe. (B) RNA blotting of PLDγs using the coding region of <i>PLD</i>γ<i>1</i> (<i>upper panel</i>) or <i>PLD</i>γ<i>2-</i>specific 5′-UTR (<i>lower panel</i>) under Al stress. Two week-old wild-type seedlings grown in ½ MS medium were treated with 100 µM AlCl<sub>3</sub>, and roots of seedlings were collected at the indicated time for RNA extraction. [α-<sup>32</sup>P]-Labeled <i>PLD</i>γ<i>1</i> cDNA or <i>PLD</i>γ<i>2</i>- specific 5′-UTR was used as a probe for northern blotting. EtBr-stained ribosomal RNA was used as a loading control.</p

Topics: Biochemistry, Biotechnology, Plant Biology, plds
Year: 2013
DOI identifier: 10.1371/journal.pone.0028086.g001
OAI identifier: oai:figshare.com:article/375331
Provided by: FigShare
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