Expression of PLDs in response to various stresses.

Abstract

<p>(A) RNA blotting of <i>PLD</i>α<i>1</i> and <i>PLD</i>γ transcripts in Arabidopsis leaves exposed to different stress treatments. Arabidopsis Col-0 (3–week old) leaves were detached and floated on water without or with indicated concentrations of chemicals, 1% NaCl, 0.6 M mannitol, 0.2 mM AlCl₃, 0.2 mM CdCl₂, 2 mM H₂O₂, 0.1 mM salicylic acid (SA), 0.1 mM methyl salicylate (MeSA), 0.1 mM abscisic acid (ABA), or 0.1 mM methyl jasmonate (MeJA) in a growth chamber at 22°C. Leaves were collected at the indicated times for RNA extraction and northern blotting with [α-³²P]-labeled <i>PLD</i>α<i>1</i> or <i>PLD</i>γ<i>1</i> full-length cDNA as a probe. (B) RNA blotting of PLDγs using the coding region of <i>PLD</i>γ<i>1</i> (<i>upper panel</i>) or <i>PLD</i>γ<i>2-</i>specific 5′-UTR (<i>lower panel</i>) under Al stress. Two week-old wild-type seedlings grown in ½ MS medium were treated with 100 µM AlCl₃, and roots of seedlings were collected at the indicated time for RNA extraction. [α-³²P]-Labeled <i>PLD</i>γ<i>1</i> cDNA or <i>PLD</i>γ<i>2</i>- specific 5′-UTR was used as a probe for northern blotting. EtBr-stained ribosomal RNA was used as a loading control.</p