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Addition of E64 restores Δ<i>srv in vitro</i> biofilm formation to wild-type levels.

By Kristie L. Connolly (338016), Amy K. Braden (338017), Robert C. Holder (338018) and Sean D. Reid (338020)

Abstract

<p>E64 (100 µM) was added to each well at the time of seeding of wild-type and Δ<i>srv</i> RGAS053, MGAS315, and MGAS5005 24 h biofilms. E64 restored biofilm formation of all Δ<i>srv</i> strains to wild-type levels. RGAS053 biofilm formation was increased compared to untreated RGAS053. Each reported value for the CV assay is an average of 6 replicates and is adjusted by the dilution factor required to obtain a spectrophometric reading (OD<sub>600 nm</sub>) (*<i>p</i>≤.01, **<i>p</i>≤0.001, ***<i>p</i>≤.0001; unpaired t-test).</p

Topics: Biochemistry, Microbiology, Cell Biology, Infectious Diseases, e64, restores, biofilm, wild-type
Year: 2013
DOI identifier: 10.1371/journal.pone.0028640.g005
OAI identifier: oai:figshare.com:article/376257
Provided by: FigShare
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