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Detection of Ply specific CD127<sup>low/-</sup> FoxP3<sup>+</sup> CD4<sup>+</sup> Treg cells in CD25 enriched tonsil and blood MNC.

By Jeffrey Pido-Lopez (338468), William W. Kwok (252537), Timothy J. Mitchell (150478), Robert S. Heyderman (108590) and Neil A. Williams (159507)

Abstract

<p>CD25 enriched MNC were stained with anti- CD127 and anti FoxP3 to allow identification of Tregs and with (<b>a</b>) strepavidin-PE, (<b>b</b>) negative control Ply tetramer-PE and (<b>c</b>) Ply tetramer-PE and analysed by FACS. A typical example of a FACS plot is shown. While the CD127<sup>low/-</sup> FoxP3<sup>+</sup> CD4<sup>+</sup> Treg cells show low level staining at 0.0% with strepavidn-PE (<b>a</b>) and negative control Ply-tetramer at 0.05% (<b>b</b>), a significantly higher percentage of Treg cells are bound by Ply-tetramers at 1.96%(<b>c</b>).</p

Topics: Microbiology, Immunology, ply, treg, cells, cd25, enriched, tonsil
Year: 2013
DOI identifier: 10.1371/journal.ppat.1002396.g007
OAI identifier: oai:figshare.com:article/378524
Provided by: FigShare
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