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HPV E7 inhibition and Rb overexpression affects the activity of the Kpnβ1 and Kpnα2 promoters.

By Pauline J. van der Watt (339036), Ellen Ngarande (339038) and Virna D. Leaner (339040)

Abstract

<p>A, B. HPV16 E7 siRNA was used to inhibit E7 expression in CaSki cells and Western blot analysis performed to analyse HPV E7 (A) and Rb (B) levels after HPV E7 knock-down. C, D. Kpnβ1 (C) and Kpnα2 (D) promoter activities were measured after E7 siRNA transfection, and were found to be significantly inhibited after HPV E7 inhibition. E, F. Rb was overexpressed in CaSki cells and Kpnβ1 (E) and Kpnα2 (F) promoter activities were found to be significantly inhibited. Results shown are the mean ± SE of experiments in quadruplicate performed at least two times (* p<0.05).</p

Topics: Biochemistry, Genetics, Molecular Biology, Cancer, e7, inhibition, rb, overexpression, affects
Year: 2013
DOI identifier: 10.1371/journal.pone.0027723.g005
OAI identifier: oai:figshare.com:article/381409
Provided by: FigShare
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