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Comparative levels of luciferase in mice infected with parental NYVAC versus NYVAC-C-KC.

By Karen V. Kibler (230157), Carmen E. Gomez (4474258), Beatriz Perdiguero (123011), Shukmei Wong (339845), Trung Huynh (339846), Susan Holechek (339848), William Arndt (329999), Victoria Jimenez (5652691), Ruben Gonzalez-Sanz (5652694), Karen Denzler (339851), Elias K. Haddad (141833), Ralf Wagner (143451), Rafick P. Sékaly (170038), James Tartaglia (339855), Giuseppe Pantaleo (10464), Bertram L. Jacobs (230168) and Mariano Esteban (50256)

Abstract

<p>Mice were infected IP with 2×10<sup>7</sup> plaque forming units/mouse of viruses (WR-luc, NYVAC-luc and NYVAC-KC-luc) expressing the luciferase marker and at different times several tissues were harvested, lysed and luciferase activity was determined as previously described <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0025674#pone.0025674-Gomez2" target="_blank">[12]</a>, <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0025674#pone.0025674-Rodriguez1" target="_blank">[13]</a>. Values are represented as reference luciferase units per gram of tissue.</p

Topics: Microbiology, Virology, levels, luciferase, mice, infected, nyvac
Year: 2013
DOI identifier: 10.1371/journal.pone.0025674.g008
OAI identifier: oai:figshare.com:article/385120
Provided by: FigShare
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