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Comparative levels of luciferase in mice infected with parental NYVAC versus NYVAC-C-KC.

By Karen V. Kibler (230157), Carmen E. Gomez (4474258), Beatriz Perdiguero (123011), Shukmei Wong (339845), Trung Huynh (339846), Susan Holechek (339848), William Arndt (329999), Victoria Jimenez (5652691), Ruben Gonzalez-Sanz (5652694), Karen Denzler (339851), Elias K. Haddad (141833), Ralf Wagner (143451), Rafick P. Sékaly (170038), James Tartaglia (339855), Giuseppe Pantaleo (10464), Bertram L. Jacobs (230168) and Mariano Esteban (50256)


<p>Mice were infected IP with 2×10<sup>7</sup> plaque forming units/mouse of viruses (WR-luc, NYVAC-luc and NYVAC-KC-luc) expressing the luciferase marker and at different times several tissues were harvested, lysed and luciferase activity was determined as previously described <a href="" target="_blank">[12]</a>, <a href="" target="_blank">[13]</a>. Values are represented as reference luciferase units per gram of tissue.</p

Topics: Microbiology, Virology, levels, luciferase, mice, infected, nyvac
Year: 2013
DOI identifier: 10.1371/journal.pone.0025674.g008
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Provided by: FigShare
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