Expression of <i>Met</i>, <i>gce</i> and <i>Kr-h1</i> is reduced in the <i>Axn</i>, <i>slmb</i> and <i>nkd</i> mutants.

Abstract

<p>(A) Total RNAs were extracted from <i>Oregon</i> R, <i>Axn^EY10228</i>, <i>slmb⁰⁰²⁹⁵</i>, and <i>nkd²</i> 2^nd instar larvae. The mRNA levels of <i>Met</i>, <i>gce</i> and <i>Kr-h1</i> were assessed by quantitative real-time PCR and normalized to <i>rp49</i> mRNA. Values shown are the means of 4 independent experiments ± standard deviations. (B) The same total RNAs described in [A] were used as the templates for a 30-cycle reverse transcriptional PCR. The RT-PCR products were analyzed by DNA agarose gel electrophoresis.</p

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