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Expression of <i>Met</i>, <i>gce</i> and <i>Kr-h1</i> is reduced in the <i>Axn</i>, <i>slmb</i> and <i>nkd</i> mutants.

By Mohamed Abdou (339977), Cheng Peng (118834), Jianhua Huang (206865), Ola Zyaan (339978), Sheng Wang (339979), Sheng Li (107261) and Jian Wang (5901)

Abstract

<p>(A) Total RNAs were extracted from <i>Oregon</i> R, <i>Axn<sup>EY10228</sup></i>, <i>slmb<sup>00295</sup></i>, and <i>nkd<sup>2</sup></i> 2<sup>nd</sup> instar larvae. The mRNA levels of <i>Met</i>, <i>gce</i> and <i>Kr-h1</i> were assessed by quantitative real-time PCR and normalized to <i>rp49</i> mRNA. Values shown are the means of 4 independent experiments ± standard deviations. (B) The same total RNAs described in [A] were used as the templates for a 30-cycle reverse transcriptional PCR. The RT-PCR products were analyzed by DNA agarose gel electrophoresis.</p

Topics: Genetics, Molecular Biology, Developmental Biology, reduced
Year: 2013
DOI identifier: 10.1371/journal.pone.0026772.g007
OAI identifier: oai:figshare.com:article/385678
Provided by: FigShare
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