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<i>Shigella dysenteriae</i> adherence and invasion of human epithelial cells by Transmission Electron Microscopy.

By Radhakrishnan Prakash (340232), Subramaniya Bharathi Raja (340233), Halagowder Devaraj (340234) and Sivasitambaram Niranjali Devaraj (340235)

Abstract

<p>After 1 h and 2 h of infection, cells were fixed and processed for Transmission Electron Microscopy analysis as described in materials and <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0027046#s2" target="_blank">methods</a>. Control cells showed intact nuclei and other cell organelles (a). 1 h of infection of HT 29 cells with <i>Shigellae</i> showed the adherence and pseudopod projection formation (b). Endocytic processes and phagocytosis of bacteria was seen in the vacuoles of HT 29 cells at the end of 2 h infection (c). The scale bars indicate 1 µm. [M – Mucin; A – Adherence; P – Pseudopod formation; I - Invasion].</p

Topics: Biochemistry, Cell Biology, Biotechnology, Chemistry, Immunology, Virology, adherence, epithelial, cells, electron
Year: 2013
DOI identifier: 10.1371/journal.pone.0027046.g006
OAI identifier: oai:figshare.com:article/386764
Provided by: FigShare
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