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Knocking down GLUT 5 mRNA in MCF7 cells has no effect on fructose uptake.

By Gayatri Gowrishankar (340478), Sabine Zitzmann-Kolbe (340479), Anitha Junutula (340480), Robert Reeves (340481), Jelena Levi (340482), Ananth Srinivasan (340484), Kjerstin Bruus-Jensen (340486), John Cyr (340487), Ludger Dinkelborg (340488) and Sanjiv S. Gambhir (163568)

Abstract

<p>GLUT 5 specific siRNA and negative control siRNA were transfected into MCF7 cells. 48 hrs after transfection cells were simultaneously interrogated for both mRNA levels (using quantitative real time PCR) (right Y axis) and for fructose uptake (left Y axis) by incubating with C14 labeled fructose. The error bars represent S.E.M of triplicates.</p

Topics: Biochemistry, Cell Biology, Genetics, Molecular Biology, Biological Sciences, Cancer, glut, mrna, mcf7, cells, fructose
Year: 2013
DOI identifier: 10.1371/journal.pone.0026902.g003
OAI identifier: oai:figshare.com:article/387471
Provided by: FigShare
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