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Expansion of hAEC.

By Gita Pratama (340594), Vijesh Vaghjiani (158195), Jing Yang Tee (324892), Yu Han Liu (324891), James Chan (158218), Charmaine Tan (340597), Padma Murthi (158212), Caroline Gargett (340600) and Ursula Manuelpillai (133311)

Abstract

<p>The hAEC grew well in the xenobiotic-free media Epilife until passage 5 (P5) and plateaued thereafter. A similar trend was seen in control cultures grown in DMEM/F12+10% FCS but the cumulative cell number in Epilife was lower than controls (*p = 0.002; <b>A</b>). Cumulative population doubling of hAEC cultured in Epilife media was lower than DMEM/F12+10% FCS (*p = 0.0026; <b>B</b>). hAEC appeared stromal-like at P5 in Epilife and DMEM/F12+10% FCS (<b>C</b>). Stromal-like cells at P4 retained the CFSE label suggesting the stromal cells arose from the labeled P2 epithelial cells (<b>D</b>). Scale bars = 100 µm.</p

Topics: Cell Biology, Physiology, Immunology, Developmental Biology, immunology, physiology, cell biology, developmental biology
Year: 2013
DOI identifier: 10.1371/journal.pone.0026136.g001
OAI identifier: oai:figshare.com:article/387860
Provided by: FigShare
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