Expansion of hAEC.

Abstract

<p>The hAEC grew well in the xenobiotic-free media Epilife until passage 5 (P5) and plateaued thereafter. A similar trend was seen in control cultures grown in DMEM/F12+10% FCS but the cumulative cell number in Epilife was lower than controls (*p = 0.002; <b>A</b>). Cumulative population doubling of hAEC cultured in Epilife media was lower than DMEM/F12+10% FCS (*p = 0.0026; <b>B</b>). hAEC appeared stromal-like at P5 in Epilife and DMEM/F12+10% FCS (<b>C</b>). Stromal-like cells at P4 retained the CFSE label suggesting the stromal cells arose from the labeled P2 epithelial cells (<b>D</b>). Scale bars = 100 µm.</p