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Expression of vTR harboring the mutant template (AU5) decreases cell proliferation of an avian cancer cell line.

By Benedikt B. Kaufer (341018), Sina Arndt (341019), Sascha Trapp (341020), Nikolaus Osterrieder (80314) and Keith W. Jarosinski (163234)

Abstract

<p>A) Analysis of telomerase activity in primary CEC cultures, the chicken fibroblast cell line DF-1 with or without TERT expression, and the quail QT35 cancer cell line using TRAP assays. TRAP products representing telomere elongation and internal control (IC) are indicated. B) RT-qPCR of vTR copies in polyclonal empty vector, vTR or AU5 QT35 cell lines induced with 1 µg/ml doxycycline (Dox) for 3 and 5 d or left uninduced. Data is shown as relative quantitation (RQ) of vTR copies relative to quail GAPDH RNA copies that served as an endogenous control. C) Percent (%) confluency of vector, vTR, and AU5 cell lines over the course of 31 d. Results are shown as means and standard errors of three independent experiments. <i>P</i> values were determined between each group using Student's <i>t</i> tests.</p

Topics: Infectious Diseases, vtr, harboring, mutant, template, decreases, proliferation, avian, cancer
Year: 2013
DOI identifier: 10.1371/journal.ppat.1002333.g002
OAI identifier: oai:figshare.com:article/389772
Provided by: FigShare
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