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Effects on gene expression of continuous exposure of mineralizing NHBC cultures to rhSCL.

By Asiri R. Wijenayaka (342602), Masakazu Kogawa (342603), Hui Peng Lim (342605), Lynda F. Bonewald (342609), David M. Findlay (342610) and Gerald J. Atkins (342612)

Abstract

<p>NHBC were cultured under mineralizing conditions for up to 35 days in the absence or presence of rhSCL at 50 ng/ml. Media were replenished every 3–4 days. At the time points indicated, total RNA was prepared and real-time RT-PCR performed to determine mRNA expression of A) <i>DMP1</i>, B) <i>SOST</i>, C) <i>OPG</i> and D) <i>RANKL</i>. Data shown are means of triplicate reactions ± SD normalized to expression of 18S mRNA. Significant differences to untreated control are indicated by * <i>p</i><0.05 and *** <i>p</i><0.001. Similar results were obtained from 3 independent experiments using NHBC from different donors.</p

Topics: Biochemistry, Cell Biology, Physiology, Chemistry, Ecology, Mathematics, mineralizing, nhbc, cultures
Year: 2013
DOI identifier: 10.1371/journal.pone.0025900.g001
OAI identifier: oai:figshare.com:article/397868
Provided by: FigShare
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