Effects on gene expression of continuous exposure of mineralizing NHBC cultures to rhSCL.

Abstract

<p>NHBC were cultured under mineralizing conditions for up to 35 days in the absence or presence of rhSCL at 50 ng/ml. Media were replenished every 3–4 days. At the time points indicated, total RNA was prepared and real-time RT-PCR performed to determine mRNA expression of A) <i>DMP1</i>, B) <i>SOST</i>, C) <i>OPG</i> and D) <i>RANKL</i>. Data shown are means of triplicate reactions ± SD normalized to expression of 18S mRNA. Significant differences to untreated control are indicated by * <i>p</i><0.05 and *** <i>p</i><0.001. Similar results were obtained from 3 independent experiments using NHBC from different donors.</p

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