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Genes in the region of the <i>igaB</i> insertion site.

By Timothy F. Murphy (308701), Alan J. Lesse (342703), Charmaine Kirkham (342705), Huachun Zhong (342706), Sanjay Sethi (95853) and Robert S. Munson Jr (342708)

Abstract

<p>A. Map of genes from ORFs HI0163 through HI0185 (not to scale) in <i>H. influenzae</i> strain Rd KW20 which does not contain the <i>igaB</i> gene. The dotted line represents ORFs HI0167 through HI0182. Horizontal arrows indicate location of oligonucleotide primers used in PCR reactions. B. Ethidium bromide stained agarose gels showing results of PCR reactions with genomic DNA of <i>H. influenzae</i> strains that do not contain the <i>igaB</i> gene with the oligonucleotide primers noted in A. Lanes contain genomic DNA from strains a. Rd KW20, b. 11P6H (which contains <i>igaB</i>), c. 5P28H1, d. 14P14H1, e. 31P7H1, f. 32P8H1, g. 48P28H1, h. 56P76H1, i. 1862, j. 3222B. Molecular size standards are noted in kilobases.</p

Topics: Medicine, Microbiology, Genetics, Infectious Diseases, insertion
Year: 2013
DOI identifier: 10.1371/journal.pone.0025923.g002
OAI identifier: oai:figshare.com:article/398235
Provided by: FigShare
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