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Both the proteasome 19S regulatory particle and 20S core particle associate with actin filaments.

By Brian Haarer (343459), Dimitra Aggeli (343460), Susan Viggiano (343461), Daniel J. Burke (272448) and David C. Amberg (343462)

Abstract

<p>4 µM yeast actin and either ∼65 nM 19S proteasome regulatory particle (A and B) or 50–100 nM 20S proteasome core particle (C and D) were incubated in G-buffer (A and C) or in F-buffer (C and D), centrifuged at 190,000×g and volume-corrected samples were taken from the supernatant (S) or pellet (P) phases. T are the fractions taken prior to centrifugation. All fractions were separated by SDS-PAGE and stained with SYPRO Ruby (Bio-Rad, Hercules CA).</p

Topics: Biochemistry, Genetics, proteasome, 19s, 20s, actin
Year: 2013
DOI identifier: 10.1371/journal.pgen.1002288.g008
OAI identifier: oai:figshare.com:article/402899
Provided by: FigShare
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