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The effect of SOD3 on Erk1/2 signaling pathway.

By Lilja E. Laatikainen (345194), Mariarosaria Incoronato (197260), Maria Domenica Castellone (345195), Juha P. Laurila (345197), Massimo Santoro (141869) and Mikko O. Laukkanen (197270)

Abstract

<p>(A) Western blot analysis showed increased Erk1/2 phosphorylation in <i>sod3</i> transduced muscles at 3-day, 7-day, and 10-day time points (p<0.001 (***), p<0.05 (*), and p<0.001 (***), respectively) as compared to corresponding <i>lacz</i> controls. The samples were normalized with total Erk1/2 antibody. Erk1/2 activation was supported by significant up-regulation of its downstream transcription factors (B) <i>elk-1</i> (p<0.01 (**)) and (C) <i>ets-1</i> (p<0.001 (***)), measured by mRNA synthesis in <i>sod3</i> animals as compared to LacZ control tissues. The injury itself moderately increased production of both molecules as compared to normal uninjured tissue control. However, the significant difference between normal control tissue and LacZ control (p<0.05 (*)) was achieved only in <i>ets-1</i> expression. (D) <i>Sod3</i> transduced muscles have increased levels of Akt phosphorylation only at 3-day time point (p<0.001 (***)). The samples were normalized with total Akt antibody.</p

Topics: Cell Biology, Molecular Biology, sod3, signaling
Year: 2013
DOI identifier: 10.1371/journal.pone.0024456.g002
OAI identifier: oai:figshare.com:article/410973
Provided by: FigShare
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