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Upregulation of Helios expression by Teff cells in suppression assays.

By Tatiana Akimova (345316), Ulf H. Beier (188290), Liqing Wang (188282), Matthew H. Levine (345319) and Wayne W. Hancock (44791)

Abstract

<p>(A) Analysis of CD4+ gated murine Tregs and Teffs after 3 days of suppression assay showed that while Tregs gradually lose Foxp3 (top) and Helios (bottom) expression, a substantial proportion of Teff cells upregulate Helios (bottom), but not Foxp3 (top) expression; CFSE-negative gates show Tregs. (B) Treg division during suppression assays correlated with Helios and Foxp3 co-expression, while non-dividing Tregs lost both markers. Upper row: gating of dividing and non-dividing Tregs, with numbers showing % of Treg divisions at 1/1 (left), ½ (middle) and ¼ (right) ratios; arrows point to Helios and Foxp3 co-expression in corresponding divided and non-divided Tregs subsets through the same 1/1 to ¼ Treg/Teff ratios. (C) Tregs do not induce Helios expression in Teffs since CFSE+ CD4+ Teffs have similar Helios expression despite differing numbers of Tregs (1/1, ½ and ¼ ratios shown). (D) Analysis of Teffs gated into Helios+ and Helios- subsets showed Helios+ Teffs have a higher division rate and greater resistance to Treg suppression than Helios- mouse Teffs. Human Helios+ CD4+ (E) and Helios+ CD8 (F) Teffs showed higher divisions than Helios- cells regardless of Treg presence or absence, and Helios+ responder cells were more resistant to suppression than Helios- responders. Data are representative of 3 experiments.</p

Topics: Cell Biology, Immunology, helios, teff, cells, suppression
Year: 2013
DOI identifier: 10.1371/journal.pone.0024226.g003
OAI identifier: oai:figshare.com:article/411745
Provided by: FigShare
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