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EF24 induced cell cycle arrest and inhibited the NF-κB pathway.

By Yingjian Liang (300784), Dalong Yin (345445), Limin Hou (345446), Tongsen Zheng (345447), Jiabei Wang (345448), Xianzhi Meng (345449), Zhaoyang Lu (345450), Xuan Song (345451), Shangha Pan (201039), Hongchi Jiang (201045) and Lianxin Liu (300790)

Abstract

<p>(<b>A</b>). DNA content (propidium iodide) and cell cycle analysis of EF24-treated cells. The five HCC cells were incubated with 0, 2 µmol/L EF24 for 48 h. The numbers of cells in G0/G1 phase, S phase and G2/M phase was determined via flow cytometry. (<b>B</b>). Representative histograms from cytometrically analyzed the five cell lines treated with control and EF24. Assay was done in triplicate and p<0.05 is denoted by “*”. (<b>C</b>). Expression of G<sub>2</sub>/M cell cycle relative proteins Tyr15 and Thr161 phosphorylation of cdc2 and Cyclin B1 were determined via western blot after treatment with 2 and 4 µmol/L EF24 for 24 h. β-actin was used as the internal control. (<b>D</b>). Nuclear extracts were prepared and subjected to EMSA to measure NF-κB DNA-binding activity. (<b>E</b>). Expression of p65, Bcl-2, Bax and COX-2 via western blot.</p

Topics: Cell Biology, Molecular Biology, Pharmacology, Cancer, induced, inhibited
Year: 2013
DOI identifier: 10.1371/journal.pone.0023908.g002
OAI identifier: oai:figshare.com:article/412357
Provided by: FigShare
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