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Phenotypic assessment of memory conventional and regulatory CD4<sup>+</sup> T-cell subsets in circulating lymphocytes of healthy donors and EOC patients.

By Nassima Redjimi (347000), Karine Duperrier-Amouriaux (216997), Isabelle Raimbaud (217007), Immanuel Luescher (347001), Danijel Dojcinovic (347002), Jean-Marc Classe (217015), Dominique Berton-Rigaud (347003), Jean-Sébastien Frenel (347005), Emmanuelle Bourbouloux (347007), Danila Valmori (57332) and Maha Ayyoub (57333)

Abstract

<p><b>A</b>. CD4<sup>+</sup> T cells were stained with anti-FOXP3, -CD25, -CD45RA and CD127 antibodies and analyzed by flow cytometry. Expression of CD25 and CD127 defines 3 populations of memory (CD45RA<sup>−</sup>) CD4<sup>+</sup> T cells: conventional CD25<sup>−</sup>CD127<sup>+</sup> and CD25<sup>−</sup>CD127<sup>−</sup> and Treg CD25<sup>+</sup>CD127<sup>−</sup> (left dot plot, numbers correspond to the proportion of each subset among memory CD4<sup>+</sup> T cells). Histograms show the expression of FOXP3 in the defined memory CD4<sup>+</sup> T-cell subsets. <b>B</b>. The proportion of conventional CD25<sup>−</sup>CD127<sup>+</sup> and CD25<sup>−</sup>CD127<sup>−</sup> and Treg CD25<sup>+</sup>CD127<sup>−</sup> subsets, defined in <b>A</b>, among memory CD4<sup>+</sup> T cells of healthy donors (HD, n = 27) and patients (P, n = 18).</p

Topics: Physiology, Immunology, Cancer, t-cell, subsets, circulating, lymphocytes, donors, eoc
Year: 2013
DOI identifier: 10.1371/journal.pone.0022845.g001
OAI identifier: oai:figshare.com:article/420499
Provided by: FigShare
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