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Dynamic GFP expression pattern in liver morphogenesis of <i>Tg(Apo14: GFP)</i> from 2dpf to 7dpf.

By Rui Wang (52434), Zhi Li (72309), Yang Wang (5921) and Jian-Fang Gui (259030)

Abstract

<p>(<b>a, e, i, m, q</b>) show the left lateral view of <i>Tg(Apo14: GFP)</i> larvae from 2dpf to 7dpf. (<b>b, f, j, n, r</b>) show the cross sections through intestine bulb and liver of the larvae at same stage as the left columns. Scale bar: 150 µm. (<b>c, g, k, o, s</b>) are magnifications of the corresponding frame areas in (b, f, j, n, r), which highlight the GFP expression in the hepatocytes. Scale bar: 30 µm. (<b>d, h, l, p</b>) are magnifications of the corresponding circle areas in (b, f, j, n), which highlight the GFP expression on the YSL. Scale bar: 30 µm. Green fluorescence is emitted from the <i>Apo-14</i> promoter-driven GFP, and red fluorescence is stained by PI for showing the nuclear position. Arrowheads point to hepatocytes, arrows mark sinusoids, and the asterisks delineate the GFP-expressed nuclei on YSL. Li: liver; In: intestine bulb; Pa: pancreas.</p

Topics: Biotechnology, Developmental Biology, gfp, morphogenesis, 2dpf
Year: 2013
DOI identifier: 10.1371/journal.pone.0022555.g005
OAI identifier: oai:figshare.com:article/424456
Provided by: FigShare
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