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Identification of the <i>AtBMI1C</i> mutant and characterization of artificial microRNAi lines.

By Wei Li (7081), Zheng Wang (25883), Jian Li (41607), Hongchun Yang (170145), Sujuan Cui (170199), Xiaoxue Wang (311457) and Ligeng Ma (170211)

Abstract

<p>(A) Genomic architecture of <i>AtBMI1C</i> and position of the mutation in <i>atbmi1c-1</i>. The 5′ or 3′ UTR is represented by a gray bar. Exons are represented by black bars. Introns are represented by black lines. The T-DNA insertion in <i>atbmi1c-1</i> (SALK_148143) is located in the 5′ UTR of <i>AtBMI1C</i>. Scale bar, 500 bp. (B) Detection of <i>AtBMI1C</i> mRNA in a homozygous <i>atbmi1c-1</i> T-DNA insertion line by semiquantitative RT-PCR. Total RNA was extracted from the inflorescences of homozygous <i>atbmi1c</i> and wild-type plants. Semiquantitative RT-PCR was performed to amplify the full-length transcript using <i>ACTIN2/7</i> as an endogenous control. (C) Characterization of <i>AtBMI1C</i> mRNA abundance in AtBMI1C-Rs. Total RNA was extracted from the inflorescences of AtBMI1C-Rs and wild-type plants. Semiquantitative RT-PCR was conducted to amplify the full-length transcript using <i>ACTIN2/7</i> as an endogenous control. (D) Morphology of the AtBMI1C-Rs, in which <i>AtBMI1C</i> was down-regulated, compared to wild type and AtBMI1C-R12, an amiRNAi line in which the expression of <i>AtBMI1C</i> was almost the same as in wild type. (E) Flowering time in the AtBMI1C-Rs was the same as in wild type. Plants were grown under LD conditions. The number of rosette leaves was determined after bolting.</p

Topics: Genetics, Developmental Biology, Plant Biology, mutant, characterization, micrornai
Year: 2013
DOI identifier: 10.1371/journal.pone.0021364.g004
OAI identifier: oai:figshare.com:article/434908
Provided by: FigShare
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