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Increased kyn release of SKOV-3 cells upon 1-D-MT treatment.

By Christiane A. Opitz (351069), Ulrike M. Litzenburger (126790), Uta Opitz (351071), Felix Sahm (126791), Katharina Ochs (351073), Christian Lutz (351075), Wolfgang Wick (126794) and Michael Platten (34815)

Abstract

<p>(<b>A</b>) Kyn concentrations released by SKOV-3 cells after treatment with 1-D-MT (white circles), 1-L-MT (black circles) and the racemic mixture of 1-MT (black triangles) measured after 48 h by HPLC. (<b>B</b>) Kyn release of SKOV-3 cells in response to 500 µM 1-D-MT in the presence of increasing trp concentrations. (<b>C</b>) Kyn release of SKOV-3 cells treated with different concentrations of trp alone (open circles) or in combination with 1 mM 1-D-MT (filled circles) measured after 48 h by HPLC. (<b>D</b>) Kyn production in IDO1 enzymatic assays performed in the presence of 100 µM trp in combination with increasing 1-D-MT concentrations. (<b>E</b>) Kyn production of IDO1 enzyme in the presence of increasing concentrations of trp alone (open circles) or in combination with 1-D-MT (filled circles). Experiments were performed in triplicate. Data are mean ± SEM. * (p<0.05).</p

Topics: Biochemistry, Molecular Biology, Pharmacology, Chemistry, Immunology, Cancer, kyn, skov-3, cells, 1-d-mt
Year: 2013
DOI identifier: 10.1371/journal.pone.0019823.g004
OAI identifier: oai:figshare.com:article/443309
Provided by: FigShare
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