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Immunodetection of PrPSc oligomers/multimers with PRIOC mAbs by Sandwich ELISA.

By Mourad Tayebi (41958), Daryl Rhys Jones (351314), William Alexander Taylor (351316), Benjamin Frederick Stileman (351319), Charlotte Chapman (351321), Deming Zhao (334103) and Monique David (351322)

Abstract

<p>(<b>A</b>). Principle of a newly developed specific and sensitive ELISA designed for the detection of PrPSc oligomers/multimers. (<b>B</b>). RML-infected brain homogenates were used to assess specific binding of PRIOC mAbs to PrPSc oligomers following proteinase K (PK) digestion. 50 µl of 5 µg/ml of PRIOC or anti-monomer antibody (AB) was used to coat the ELISA plate in coating buffer. RML-infected brain homogenate was added to the wells followed by a biotinylated PRIOC mAb or anti-monomer antibody (AB). The sandwich format of the assay has established the specificity of PRIOC antibody for post-PK PrPSc oligomers. Error bars represent the mean antibody level derived from n = 4 wells.</p

Topics: Biochemistry, Neuroscience, Immunology, Infectious Diseases, prpsc, prioc, mabs
Year: 2013
DOI identifier: 10.1371/journal.pone.0019998.g007
OAI identifier: oai:figshare.com:article/444651
Provided by: FigShare
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