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Caffeine elicits the increases of intracellular Ca<sup>2+</sup> fluorescence intensity in cerebral VSMCs isolated from CON-28 d, SUS-28 d, and SUS+Rec-3 d rats.

By Jun-Hui Xue (351390), Lian-Hong Chen (351391), Hua-Zhou Zhao (351392), Yong-Dong Pu (351394), Han-Zhong Feng (351397), Yu-Guang Ma (321921), Jin Ma (335456), Yao-Ming Chang (335457), Zuo-Ming Zhang (351402) and Man-Jiang Xie (321931)

Abstract

<p>(A) Representative dot graph of Fluo-3/AM fluorescence recorded showed the typical transient increases of intracellular Ca<sup>2+</sup> fluorescence intensity evoked by 10 mM caffeine. Caffeine was present at times shown by the horizontal bar. (B) Summarized data indicated the average changes of the intracellular Ca<sup>2+</sup> fluorescence intensity before and during the application of caffeine. Values are means ± SE with the number of cells recorded in parentheses. CON-28 d: 28-day simultaneous control rats, SUS-28 d: 28-day tail-suspended rats, SUS+Rec-3 d: tail-suspended rats submitted to a recovery period of 3 days at the end of 28-day suspension. *<i>P</i><0.05 as compared with their controls by t-test, respectively.</p

Topics: Biochemistry, Molecular Biology, Neuroscience, Physiology, elicits, increases, intracellular, fluorescence, cerebral, vsmcs, con-28, sus-28
Year: 2013
DOI identifier: 10.1371/journal.pone.0019775.g004
OAI identifier: oai:figshare.com:article/444800
Provided by: FigShare
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