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FISH detection of <i>Wolbachia</i> in circulating haemocytes and haematopoietic organs.

By Frédéric Chevalier (353027), Juline Herbinière-Gaboreau (353028), Joanne Bertaux (353029), Maryline Raimond (146134), Franck Morel (353030), Didier Bouchon (146143), Pierre Grève (353031) and Christine Braquart-Varnier (146129)

Abstract

<p>In infected animals, <i>Wolbachia</i> (in red) colonized many haemocytes (<b>A</b>, <b>B</b>) and the central area (*) as well as the cortex (**) of the haematopoietic organ (<b>C</b>), although groups of cells remained uncolonized (Arrowheads, <b>D</b>–<b>F</b>). The control uninfected animals presented only rare <i>Wolbachia</i>-like artefacts (haemocytes, <b>G–H</b>, haematopoietic organ, <b>I</b>). <b>A</b>–<b>C, G–H</b>: red: <i>Wolbachia</i>, green: Actin; blue: Nuclei. <b>A</b>, <b>B</b>, <b>G</b>, <b>H</b>: average intensity Z-projections. <b>B</b> and <b>H</b>: Close-ups. <b>D</b>–<b>F</b>: 3D analysis (ImageJ 3D viewer) of image <b>C</b>. <b>D</b>: tilted volume rendering of <i>Wolbachia</i> (red) and the nuclei (turquoise) in the central area extracted from the Z-stack. <b>E</b> and <b>F</b>: volume rendering of <i>Wolbachia</i> (grey-scale) from the whole haematopoietic organ. <b>E</b>: front view corresponding to image <b>C</b>, <b>F</b>: tilted view (180°).</p

Topics: Microbiology, Cell Biology, Immunology, detection, circulating, haemocytes, haematopoietic
Year: 2013
DOI identifier: 10.1371/journal.pone.0018531.g007
OAI identifier: oai:figshare.com:article/453078
Provided by: FigShare
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