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Radiolabeling of rCR2.

By Adam Badar (353606), Sarah DeFreitas (353608), James M. McDonnell (353610), Norhakim Yahya (353612), David Thakor (353613), Reza Razavi (90384), Richard Smith (45265), Steven Sacks (353615) and Gregory E. D. Mullen (67161)


<p>(<b><i>A</i></b>) rCR2 was incubated with [<sup>99m</sup>Tc(CO)3(OH2)3]<sup>+</sup> at 37°C for 30 minutes at a protein concentration of 1 µg/µL and analysed by instant thin layer chromatography (ITLC). The radiolabeling efficiency was calculated as a ratio of the radiolabeled protein peak integral (R<sub>f</sub> = 0) to the unincorporated [<sup>99m</sup>Tc(CO)<sub>3</sub>(OH<sub>2</sub>)<sub>3</sub>]<sup>+</sup> peak integral (R<sub>f</sub> = 1.0). (<b><i>B</i></b>) The radiolabeling efficiency was followed over a 120 minute period. Aliquots were removed periodically and analysed by ITLC and the radiolabeling efficiency was determined over time.</p

Topics: Chemistry, Immunology, immunology, chemistry
Year: 2013
DOI identifier: 10.1371/journal.pone.0018275.g006
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Provided by: FigShare
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