Radiolabeling of rCR2.

Abstract

<p>(<b><i>A</i></b>) rCR2 was incubated with [^99mTc(CO)3(OH2)3]⁺ at 37°C for 30 minutes at a protein concentration of 1 µg/µL and analysed by instant thin layer chromatography (ITLC). The radiolabeling efficiency was calculated as a ratio of the radiolabeled protein peak integral (R_f = 0) to the unincorporated [^99mTc(CO)₃(OH₂)₃]⁺ peak integral (R_f = 1.0). (<b><i>B</i></b>) The radiolabeling efficiency was followed over a 120 minute period. Aliquots were removed periodically and analysed by ITLC and the radiolabeling efficiency was determined over time.</p

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