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Specificity of HIF-1α dependent activation of the DDX3 gene.

By Mahendran Botlagunta (354370), Balaji Krishnamachary (309844), Farhad Vesuna (354371), Paul T. Winnard Jr. (354373), Guus M. Bol (354375), Arvind H. Patel (33806) and Venu Raman (309847)


<p>Stable MCF 10A and MCF 7-HIF-1α shRNA clones were generated. (a) MCF 10A-shHIF-1α and (b) MCF 7-shHIF-1α cells were subjected to 200 µM CoCl<sub>2</sub> for the indicated times. qRT-PCR analysis was performed using specific primers for human DDX3, HIF-1α and HIF-2α. DDX3, HIF-1α, and HIF-2α protein levels in cell lysates of MCF 10A-shHIF-1α (c) and MCF 7-shHIF-1α (d) cells were examined by immunoblots using protein specific antibodies. GAPDH served as a loading control. Error bars represent ±SD.</p

Topics: Genetics, Molecular Biology, Cancer, activation, ddx3
Year: 2013
DOI identifier: 10.1371/journal.pone.0017563.g002
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Provided by: FigShare
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