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Inhibitory effects of <i>focA</i>-positive and <i>focA</i>-negative <i>E. coli</i> isolates on <i>Salmonella Typhimurium</i> invasion.

By Peter Schierack (355803), Sylvia Kleta (355804), Karsten Tedin (355806), Julius Tachu Babila (355807), Sibylle Oswald (355808), Tobias A. Oelschlaeger (281562), Rico Hiemann (355810), Susanne Paetzold (355813) and Lothar H. Wieler (106662)


<p>Confluent monolayers of IPEC-J2 cells were pre-incubated with <i>E. coli</i> Nissle 1917 (EcN, <i>focA</i>-positive strain), <i>E. coli</i> WS15C1 (<i>focA</i>-positive strain), <i>E. coli</i> WS30C1 (<i>focA</i>-negative strain) and <i>E. coli</i> WS46C1 (<i>focA</i>-negative strain) using an MOI of 100∶1 <i>E. coli</i> to host cells. After two hours, cells were washed and adhesion efficiencies of <i>E. coli</i> isolates were determined (left side); alternatively, cells were washed after two hours and infected with <i>Salmonella Typhimurium</i> using an MOI of 100∶1 <i>Salmonella</i> to host cells (right side). Adhesion levels in percent (%) were expressed relative to adhesion of EcN. Invasion levels in percent (%) were expressed relative to <i>Salmonella</i> invasion without pre-incubation with bacteria (<i>Salmonella</i> mono-infection). The data are the mean ± S.E.M. of at least three separate experiments in duplicate wells. * = p<0.01 compared to EcN adhesion (left side) or <i>Salmonella</i> mono-infection (right side).</p

Topics: Engineering, Microbiology, Infectious Diseases, isolates
Year: 2013
DOI identifier: 10.1371/journal.pone.0014712.g008
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Provided by: FigShare
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