Inhibitory effects of <i>focA</i>-positive and <i>focA</i>-negative <i>E. coli</i> isolates on <i>Salmonella Typhimurium</i> invasion.

Abstract

<p>Confluent monolayers of IPEC-J2 cells were pre-incubated with <i>E. coli</i> Nissle 1917 (EcN, <i>focA</i>-positive strain), <i>E. coli</i> WS15C1 (<i>focA</i>-positive strain), <i>E. coli</i> WS30C1 (<i>focA</i>-negative strain) and <i>E. coli</i> WS46C1 (<i>focA</i>-negative strain) using an MOI of 100∶1 <i>E. coli</i> to host cells. After two hours, cells were washed and adhesion efficiencies of <i>E. coli</i> isolates were determined (left side); alternatively, cells were washed after two hours and infected with <i>Salmonella Typhimurium</i> using an MOI of 100∶1 <i>Salmonella</i> to host cells (right side). Adhesion levels in percent (%) were expressed relative to adhesion of EcN. Invasion levels in percent (%) were expressed relative to <i>Salmonella</i> invasion without pre-incubation with bacteria (<i>Salmonella</i> mono-infection). The data are the mean ± S.E.M. of at least three separate experiments in duplicate wells. * = p<0.01 compared to EcN adhesion (left side) or <i>Salmonella</i> mono-infection (right side).</p

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