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Role of c-Src and Syk downstream of integrin αIIbβ3 in thromboxane generation in platelets.

By Kamala Bhavaraju (356204), Parth R. Lakhani (356206), Robert T. Dorsam (356208), Jianguo Jin (356210), Ian S. Hitchcock (356212), Archana Sanjay (356213) and Satya P. Kunapuli (37002)

Abstract

<p>Aspirin–treated, washed platelets were stimulated with 2MeSADP (100 nM) at various time points (A) or with varying concentrations of 2MeSADP (B) for one minute under stirring conditions at 37°C. The lysates were then subjected to western blotting analysis and probed with anti-phospho-(Y416) and total c-Src antibodies as lane loading control. Washed murine (WT or c-Src KO) platelets, without aspirin-treatment, were stimulated with 2MeSADP (100 nM) for 3.5 minutes and TXB<sub>2</sub> levels were analyzed (C) as described for <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0016586#pone-0016586-g001" target="_blank">Figure 1</a>. The data are represented as the % Fold increase over the control. Aspirin-treated washed platelets were stimulated with 2MeSADP (100 nM) for (30–120 seconds) or convulxin (100 ng/ml) for 30 seconds under stirring conditions at 37°C (D). The lysates were then subjected to western blotting analysis and probed with anti- phospho- Syk(Y 525/526) and total Syk antibodies as lane loading control. The data are representative of at least 3 separate experiments.</p

Topics: Molecular Biology, Hematology, c-src, syk, downstream, integrin, thromboxane
Year: 2013
DOI identifier: 10.1371/journal.pone.0016586.g002
OAI identifier: oai:figshare.com:article/469443
Provided by: FigShare
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