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β-gal/LacZ positive cells can proliferate and contribute to myotube formation <i>in vitro</i>, and blood vessel formation <i>in vivo</i>.

By Xiaodong Mu (356545), Hairong Peng (356546), Haiying Pan (356547), Johnny Huard (192304) and Yong Li (15029)


<p>BrdU incorporation assay showed that (β-gal/LacZ)+ cells can proliferate (<b>A–C</b>). A myogenic differentiation assay, which deprives the cultures of serum, showed that (β-gal/LacZ)+ cells, in both cell populations without purification [ around 6% of cells were (β-gal/LacZ)+] (<b>D</b>) and after purification with Fluorescence-activated cell sorting (<b>E</b>), can participate in myotube formation (<b>F</b>). <i>In vivo</i>, ten days after laceration-injury of GM muscles that were transplanted with Cre-cells and Lox-cells for 3 weeks, some (β-gal/LacZ)+ signal was also found to co-localize with CD31+ signal in the blood vasculature (<b>G–L</b>). Images G–I or J–L are of the same location in tissue, and result of β-gal/LacZ staining and immunofluorescent CD31/Utrophin staining are shown here (<b>G–I</b>); arrowheads indicate β-gal+/CD31+ cells, and arrows indicate β-gal+ myofibers (<b>G–I</b>). Fluorescent co-staining of β-gal and Pax7 are also shown (<b>J–L</b>); arrowheads indicate β-gal+/Pax7+ cells, and arrows indicate CD31+ cells (<b>J–L</b>).</p

Topics: Biochemistry, Cell Biology, Physiology, Developmental Biology, cells, proliferate, myotube
Year: 2013
DOI identifier: 10.1371/journal.pone.0016699.g004
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Provided by: FigShare
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