<p>Cortical protein extracts were separated by SDS-PAGE and levels of tau phosphorylation were determined using antibodies directed at the AT8 (<i>1</i>), PHF-1 (<i>2</i>), pS262 (<i>3</i>), MC6 (<i>4</i>), and pS422 (<i>5</i>) phophoepitopes, or Total Tau (<i>6</i>). Relative immunoreactive band intensities are expressed as a percent of control (Ctl; intralipid) and are displayed for each phosphoepitope and total tau. For each condition, 1 representative datum is displayed with Ctl (n = 6), 0.5 h (n = 6), and 2 h (n = 7). Data are expressed as mean ± SD. *, and ** and denote <i>P</i><0.05, and <i>P</i><0.01 vs. Ctl, respectively; ANOVA with Newman-Keuls <i>post hoc</i> test.</p
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