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Enhanced Wnt-signaling and differentiation of CBC cells in <i>vABKO</i> and <i>vTKO</i> mice.

By Gwanghee Lee (271155), Sofia Origanti (357109), Lynn S. White (357111), Jinwu Sun (357114), Thaddeus S. Stappenbeck (357117) and Helen Piwnica-Worms (251639)


<p>(<b>A</b>) Tissue sections of small intestines prepared from <i>Vil-Cre-ER<sup>T2</sup></i> (left panel), <i>vABKO</i> (middle panel) and <i>vTKO</i> (right panel) mice were stained with antibodies specific for beta-catenin (green). Nuclei were stained with hematoxylin (blue). Green hatched lines outline nuclei. Scale bar: 20 µm. (<b>B</b>) Quantitation of nuclear beta-catenin staining is shown in A. Data is presented as mean +/− SEM. Asterisks in panel B indicate significantly different from vil-Cre-ER<sup>T2</sup> mice as determined by a Student's t-test. (<b>C</b>) Intestinal sections were stained with Periodic Acid Schiff/alcian blue to label Paneth cells and goblet cells from <i>Vil-Cre-ER<sup>T2</sup></i> (left panel), <i>vABKO</i> (middle panel) and <i>vTKO</i> (right panel) mice. Insets are magnifications of boxed regions shown in middle and right panel. Asterisks in left panel indicate CBC cells. Asterisks in middle and right panels indicate immature Paneth cells. Arrows in middle and right panel indicate small granules of immature Paneth cells. Scale bar: 10 µm.</p

Topics: Cell Biology, Genetics, Molecular Biology, Physiology, Developmental Biology, wnt-signaling, differentiation, cbc, cells
Year: 2013
DOI identifier: 10.1371/journal.pone.0015561.g008
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Provided by: FigShare
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