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hESC and phESC differ morphologically.

By Julie V. Harness (357819), Nikolay A. Turovets (357820), Magdalene J. Seiler (357821), Gabriel Nistor (244878), Gulsah Altun (357822), Larissa S. Agapova (357823), David Ferguson (357824), Louise C. Laurent (185206), Jeanne F. Loring (201461) and Hans S. Keirstead (244899)


<p>Conventionally-derived (a–f) and parthenote-derived (g–l) hESCs have distinct morphology and growth patterns in the undifferentiated state. High magnification and low magnification images have been provided for each colony to illustrate the slower growth of parthenote-derived colonies, irregular colony shape and lack of stromal cells (long, thin cells between colonies indicated by arrows) in the phESC cultures. Day 1 after plating (a, d, g and j). Day 2 after plating (b, e, h and k). Day 4 after plating (c, f, i and l). Scale bars represent 20 µm (a–c and g–i) and 10 µm (d–f and j–l).</p

Topics: Cell Biology, Neuroscience, Developmental Biology, phesc
Year: 2013
DOI identifier: 10.1371/journal.pone.0014499.g001
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Provided by: FigShare
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