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Sags induce increases in the proliferative levels of cognate lymphoma T cells.

By Juliana Mundiñano (306020), Paula M. Berguer (306017), Gabriel Cabrera (358444), Daniela Lorenzo (358445), Irene Nepomnaschy (358446) and Isabel Piazzon (306021)

Abstract

<p>(A–B) Proliferative response of lymphoma T cells <i>in vitro</i>. (A) Different numbers of T14 lymphoma cells were co-cultured with 0.7×10<sup>5</sup> mitomycin C-pretreated splenocytes from (•) non-infected AKR/J mice, (▪) MMTV BALB2-infected AKR/J mice or (▴) MMTV BALB14-infected AKR/J mice. The proliferative response was assessed by 3H-thymidine incorporation during the last 18 hr of a 1-day culture period. Data are expressed as the mean ± SD, n = 5, **p<0.01. (B) Different numbers of T8.2 lymphoma cells were co-cultured with 0.7×10<sup>5</sup> mitomycin C-pretreated intraperitoneal macrophages in the presence of 10 µg/ml of (▪) SEI, (▴) SEB or (•) PBS. The proliferative response was assessed by 3H-thymidine incorporation during the last 18 hr of a 1-day culture period. Data are expressed as the mean ± SD, n = 5, **p<0.01. (C) Proliferative response of lymphoma T cells <i>in vivo</i>. CFSE-stained T5 lymphoma cells (5×10<sup>6</sup>) were transferred intraperitoneally into syngeneic AKR/J mice. The mice were divided into three groups (n = 3 per group) and received an intraperitoneal injection of 25 µg of SEB, SEI or PBS. Twenty four hours later cells were collected by intraperitoneal washing and CFSE fluorescence was analyzed. Overlayed histograms depict a representative example. All the experiments were performed three times with similar results.</p

Topics: Cell Biology, Immunology, Cancer, Hematology, induce, increases, proliferative, levels, cognate, lymphoma
Year: 2013
DOI identifier: 10.1371/journal.pone.0015694.g002
OAI identifier: oai:figshare.com:article/480911
Provided by: FigShare
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