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rAAV replication in MO59K cells and 293 cell treated with wortmannin.

By Young-Kook Choi (358599), Kevin Nash (337750), Barry J. Byrne (321632), Nicholas Muzyczka (358600) and Sihong Song (358601)


<p>Hirt DNA was purified two days after viral infection and subjected to Southern blot analysis. All samples were triplicated and hybridized with <sup>32</sup>P labeled CMV probe. Replicated forms of rAAV include double-stranded monomer (about 3.4 kb), or dimer (about 6.8 kb), and concatamers DNA (high molecular weight). Notice that treatment of wortmannin reduced rAAV replication in a dose-dependent manner in both MO59K cells (A) and in 293 cells (B).</p

Topics: Microbiology, Virology, replication, mo59k, cells, 293, treated
Year: 2013
DOI identifier: 10.1371/journal.pone.0015073.g001
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Provided by: FigShare
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