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2-DE image of 10 µL human serum and mass spectra of glycopeptides of 2-DE separated α1-antitrypsin.

By Piliang Hao (230313), Yan Ren (149348) and Yongming Xie (263091)


<p>(A) 2-DE image of 10 µL human serum. Two largest spots of α1-antitrypsin were chosen for glycosylation analysis and named as A1PI-1 and A1PI-2. (B)–(C): Mass spectra of the glycopeptides of A1PI-1 and A1PI-2 in the mass range of 5500-7000 Dalton. Data were shown in average value. Compared with A1PI-1, the relative abundance of diantennary N-glycans indicated by arrows in A1PI-2 increased significantly, while the triantennary N-glycans decreased significantly.</p

Topics: Biochemistry, 10, serum, spectra, glycopeptides, 2-de, separated
Year: 2013
DOI identifier: 10.1371/journal.pone.0015096.g004
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Provided by: FigShare
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