The TCRα LCR drives significant mRNA expression levels of a 3′-linked HLA-B7 reporter gene in B cells.

Stefan Knirr (359521); Janette Gomos-Klein (359523); Blanca E. Andino (359524); Faith Harrow (359525); Karl F. Erhard (359526); Damian Kovalovsky (254810); Derek B. Sant'Angelo (254823); Benjamin D. Ortiz (254816)

doi:10.1371/journal.pone.0015527.g005

The TCRα LCR drives significant mRNA expression levels of a 3′-linked HLA-B7 reporter gene in B cells.

By Stefan Knirr (359521), Janette Gomos-Klein (359523), Blanca E. Andino (359524), Faith Harrow (359525), Karl F. Erhard (359526), Damian Kovalovsky (254810), Derek B. Sant'Angelo (254823) and Benjamin D. Ortiz (254816)

Abstract

(A) Northern blot analyses of RNA prepared from thymocytes and isolated spleen B cells from three independent lines of 1:8-B7 transgenic mice. Thy = Thymus, SpB = Spleen B cells, NTG = non-transgenic. (B) Graph depicting PhosporImager analyses of HLA-B7 reporter expression levels normalized to 18S rRNA. Y-axis values are expressed relative to thymus mRNA levels (designated as 100%).</p

Topics: Genetics, Molecular Biology, Biotechnology, Immunology, Developmental Biology, lcr, drives, mrna, levels, hla-b7

Year: 2013

DOI identifier: 10.1371/journal.pone.0015527.g005

OAI identifier: oai:figshare.com:article/487384

Provided by: FigShare

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The TCRα LCR drives significant mRNA expression levels of a 3′-linked HLA-B7 reporter gene in B cells.

Abstract

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