The TCRα LCR drives significant mRNA expression levels of a 3′-linked HLA-B7 reporter gene in B cells.
<p>(<b>A</b>) Northern blot analyses of RNA prepared from thymocytes and isolated spleen B cells from three independent lines of 1:8-B7 transgenic mice. Thy = Thymus, SpB = Spleen B cells, NTG = non-transgenic. (<b>B</b>) Graph depicting PhosporImager analyses of HLA-B7 reporter expression levels normalized to 18S rRNA. Y-axis values are expressed relative to thymus mRNA levels (designated as 100%).</p
Genetics, Molecular Biology, Biotechnology, Immunology, Developmental Biology, lcr, drives, mrna, levels, hla-b7
DOI identifier: 10.1371/journal.pone.0015527.g005
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