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Increased T cell-selectivity of epigenetic hCD2 promoter chromatin activation in the dual-reporter transgene context.

By Stefan Knirr (359521), Janette Gomos-Klein (359523), Blanca E. Andino (359524), Faith Harrow (359525), Karl F. Erhard (359526), Damian Kovalovsky (254810), Derek B. Sant'Angelo (254823) and Benjamin D. Ortiz (254816)

Abstract

<p>(<b>A</b>) Chromatin immunoprecipitation assay on thymocytes and isolated spleen B cells detecting the trimethyl-lysine 4 epigenetic mark on Histone H3 from single reporter (hCD2:1-8 line 4) and dual-reporter (hCD2:1-8:B7 line 10) transgenic mice. Y-axis values represent the ratio of percent H3K4me3 marks obtained at the hCD2 promoter to that detected at the endogenous GAPDH promoter, an internal standard used here as a normalizing control. (<b>B</b>) Confirmation of ChIP results using distinct, independent lines of transgenic mice bearing single reporter (hCD2:1-8 line 29) and dual reporter (hCD2:1-8:B7 line 22) transgenes. For both experiments, the Y-axis values are derived from the formula hCD2 [H3K4me3 – IgG/input]/GAPDH [H3K4me3 – IgG/input].</p

Topics: Genetics, Molecular Biology, Biotechnology, Immunology, Developmental Biology, cell-selectivity, epigenetic, hcd2, promoter, chromatin, activation, dual-reporter, transgene
Year: 2013
DOI identifier: 10.1371/journal.pone.0015527.g007
OAI identifier: oai:figshare.com:article/487534
Provided by: FigShare
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